SAB5900201

Alpaca Anti-GFP, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: alpaca
• Qualitätsniveau: 100
• Rekombinant: expressed in (E. coli)
• Konjugat: unconjugated
• Antikörperform: (Affinity purified VHH antibody)
• Klon: GE10, monoclonal
• Assay: >95% (SDS-PAGE)
• Form: lyophilized solid
• Mol-Gew.: 13.7 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• Aufgereinigt durch: (Affinity chromatography purified via Ni-charged resin); affinity chromatography (purified via Ni-charged resin)
• Konzentration: 1 mg/mL
• Methode(n): ELISA: 1:2000-1:10000; immunofluorescence: 1:100-1:600; immunoprecipitation (IP): 0.5-4.0 μg; western blot: 1:1000-1:5000
• Farbe: colorless
• pH-Wert: ( in PBS, pH 7.4)
• Isotyp: VHH
• Protein-ID-Hinterlegungsnummer: BAG71039.1
• UniProt-Hinterlegungsnummer: A0A059PIQ0
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm

Spezifität

Green fluorescence protein (GFP) and other derivatives of GFP

Immunogen

Recombinant full length GFP protein

Anwendung

ELISA, WB, IP, IF

Verpackung

50 µg per vial / 100 µg per vial

Physikalische Form

Purified VHH protein in buffer containing 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA

Rekonstituierung

Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Lagerung und Haltbarkeit

Stable for 1 years at 4°C from date of receipt.

Sonstige Hinweise

Storage at 4°C; Do not freeze.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable