SAB5801049

Alpaca Anti-Chicken IgY single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonym(e): Recombinant Alpaca single variable domain secondary Nano antibody
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: alpaca
• Rekombinant: expressed in E. coli
• Konjugat: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: secondary antibodies
• Klon: recombinant monoclonal
• Form: lyophilized solid
• Mol-Gew.: 14 kDa
• Speziesreaktivität: Alpaca, chicken
• Methode(n): ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• Größe: 0.1 mL
• Farbe: colorless
• Isotyp: VHH
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Alpaca Anti-Chicken IgY single domain Antibody is designed to specifically detect chicken IgY. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Chicken IgY single domain Antibody detects the chicken IgY selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.
Hens egg yolk immunoglobulins IgY can be transferred from the serum of mother hen to the offspring egg yolk to acquire immunity. In the immunodiagnostic technologies, IgY is an excellent antibody for using in immunological assays involving mammalian sera, due to discriminative properties of IgY compared to mammalian IgG, as IgY does not react with the rheumatoid factor and human anti-mouse IgG antibodies do not activate the complement system and do not bind to Fc receptor. Also, they have poor cross reactivity to mammalian IgG due to immunological differences. IgY does not contain a hinge region but does contain an additional constant domain. The whole IgY molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgYcontains two heavy and light chains. The common IgY is monomeric with a molecular weight of approximately 170 kDa.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Spezifität

Fc region of chicken IgY
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Verpackung

100 μg in Glass bottle

Physikalische Form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Rekonstituierung

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Lagerung und Haltbarkeit

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Sonstige Hinweise

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317,H412
• P-Sätze: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Gefahreneinstufungen: Aquatic Chronic 3 - Skin Sens. 1
• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable