SAB5201915

Monoclonal Anti-ERp57-Atto 390 antibody produced in mouse

clone 4F9, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-PDIA3
• UNSPSC-Code: 12352203
• NACRES: NA.45

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: Atto 390 conjugate
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 4F9; monoclonal
• Beschreibung: target molecular weight: 57 kDa
• Form: buffered aqueous solution
• Speziesreaktivität: human (Tested)
• Verpackung: pkg of 100 μg
• Konzentration: 1.0 mg/mL
• Methode(n): immunofluorescence: 1:100; western blot: 1:1000
• Isotyp: IgG2b
• Protein-ID-Hinterlegungsnummer: NP_005304
• UniProt-Hinterlegungsnummer: P30101
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PDIA3(2923)

Beschreibung

Immunogen

Immunogen type: Recombinant protein
Immunogen Species: Human
Immunogen Region: See Notes section/Other
Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

Anwendung

Western blotting
Dilution 1:1000
Tissue/Cell Type Used: HeLa cell lysate

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: HeLa

Zielbeschreibung

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .

Physikalische Form

50% glycerol, 0.09% Sodium Azide

PBS pH 7.4

Angaben zur Herstellung

Derived from cell/tissue culture

Lagerung und Haltbarkeit

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

Sonstige Hinweise

Additional Product Details:
Immunogen region: Amino acids 25-505
Lot Expiration/Retest Date: Expires 1 year from purchase

Other:
Immunogen region: Amino acids 25-505

Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_005304.3

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2