SAB4200806

Anti-Ezrin antibody, Mouse monoclonal

clone 3C12, purified from hybridoma cell culture

• Synonym(e): Cytovillin, Villin-2, p81
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: 3C12, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: ~80 kDa
• Speziesreaktivität: bovine, canine, hamster, kangaroo, human, monkey, rat, mouse
• Verpackung: antibody small pack of 25 μL
• Konzentration: ~1 mg/mL
• Methode(n): immunoblotting: 1-2 μg/mL using using canine MDCK cells extract; immunofluorescence: 0.5-1 μg/mL using human epidermoid carcinoma A431 cells.
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P15311
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Ezr(22350)

Beschreibung

Allgemeine Beschreibung

Ezrin, also known as cytovillin, p81 or 80K, is a.member of the ERM proteins family composed of Ezrin, Radixin, and Moesin. These proteins are known as actin-binding proteins that link the cell membrane to the actin cytoskeleton, particularly in microvillus-bearing epithelial cells. Ezrin is present in microvilli, microspikes, and membrane ruffles of cells.
The ERM proteins family are involved in the functional expression of membrane proteins on the cell surface, integrate Rho guanosine 5′-triphosphatase (GTPase) signaling regulating cytoskeletal organization and act as protein kinase A (PKA)-anchoring proteins. Therefore, ERM proteins seem to play an important role in the membrane transport of electrolytes by ion channels and transporters.

Immunogen

The carboxy-terminal part of recombinant human ezrin

Anwendung

Monoclonal Anti-Ezrin recognizes an epitope located on the C-terminal region of Ezrin. Reactivity has been observed with human, mouse, hamster, monkey, bovine, canine, rat and rat-kangaroo origin. The antibody is recommended to use in various immunological techniques, including Immunoblotting (~80kDa), immunofluorescence, Immunohistochemistry and immunoprecipitation.

Physikalische Form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Sonstige Hinweise

This product is for R&D use only, not for drug, household, or other uses.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1