SAB4200114

Anti-EDC4 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(e): Anti-Ge-1, Anti-HEDLS (Human enhancer of decapping, (large subunit), Anti-RCD-8
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~150 kDa
• Speziesreaktivität: human
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoprecipitation (IP): 5-10 μg using ysates of HEK-293 cells over expressing human EDC4; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells over expressing human EDC4; western blot: 0.5-1 μg/mL using lysates of HEK-293 cells over expressing human EDC4
• UniProt-Hinterlegungsnummer: Q6P2E9
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... EDC4(23644) ; mouse ... Edc4(234699) ; rat ... Edc4(361399)

Beschreibung

Allgemeine Beschreibung

Enhancer of mRNA-decapping protein 4 (EDC4), also known as human enhancer of decapping large subunit (HEDLS) or Ge-1, is characterized with an N-terminal WD40 repeat and a C-terminal domain which interacts with mRNA-decapping enzyme subunit 2 (Dcp2), and mediates EDC4 oligomerization and P-body localization .

The EDC4 gene maps on human chromosome 16q22.1.

Spezifität

Anti-EDC4 (N-terminal) specifically recognizes human EDC4.

Anwendung

Anti-EDC4 (N-terminal) antibody has been used in:

• immunoprecipitation
• immunofluorescence
• immunoblotting.

Biochem./physiol. Wirkung

Human enhancer of mRNA-decapping protein 4 (EDC4) associates with hDcp2 and stimulates hDcp2 activity in vitro as well as mediating the interaction between mRNA-decapping enzyme subunit 1 & 2 (DCP1 and DCP2).

Interaction of processing-body components, EDC4 and Dcp1a is essential for the post-transcriptional regulation of interleukin (IL)-6.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable