S7025

50 bp DNA Step Ladder

for DNA electrophoresis

• Synonym(e): DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp
• UNSPSC-Code: 41105335
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100
• Form: liquid
• Verwendung: 100 uses
• Eignung: suitable for electrophoresis (DNA)
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Anwendung

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Komponenten

Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

Sonstige Hinweise

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable