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Merck

S5897

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
23151817
NACRES:
NA.52

Qualität

DNA grade
for molecular biology

Qualitätsniveau

Produktlinie

BioReagent

Form

beads

Methode(n)

DNA purification: suitable

Matrix

cross-linked dextran

Quellen

1 g swells to 9-11 mL

Perlendurchmesser

20-80 μm

Porengröße

exclusion limit (20 bp dsDNA (rA)20)

pH-Wert

2-10

Fremdaktivität

DNase, RNase, none detected

Lagertemp.

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChIKey

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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Allgemeine Beschreibung

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Anwendung

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Leistungsmerkmale und Vorteile

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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