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RAB0610

Sigma-Aldrich

Human APOB / Apolipoprotein B-100 ELISA Kit

Synonym(e):

Apo B-100, Apolipoprotein B-100

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About This Item

UNSPSC-Code:
41116158
NACRES:
NA.32

Speziesreaktivität

human

Verpackung

kit of 96 wells (12 strips x 8 wells)

Methode(n)

ELISA: suitable

Aufnahme

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.5 ng/mL
standard curve range: 2.458-600 ng/mL

Nachweisverfahren

colorimetric

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... APOB(338)

Allgemeine Beschreibung

The ApoB ELISA kit provides for the Quantitative measurement of ApoB-100 in Cell Culture Supernatants, Plasma and Serum.
The APOB (apolipoprotein B) gene is mapped to human chromosome 2p24.1. Apob protein is an amphipathic protein that exists as α- helices and β-sheets. Apob is present in the major lipoproteins : chylomicrons, low density lipoproteins and very low density lipoprotein.

Anwendung

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem./physiol. Wirkung

Apob (apolipoprotein B) functions in the low-density lipoprotein cholesterol segregation. Increased APOB gene expression is observed in non-alcoholic fatty liver disease and contributes to its pathogenesis. Reduced Apob protein is indicated in familial hypobetalipoproteinemia.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDB

  • RABSTOP3ELISA Stop Solution (Item I)SDB

  • RABWASH420X Wash Buffer (Item B)SDB

Piktogramme

Corrosion

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Met. Corr. 1

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Identification of novel APOB mutations by targeted next-generation sequencing for the molecular diagnosis of familial hypobetalipoproteinemia.
Rimbert A, et al.
Atherosclerosis, 250, 52-56 (2016)
Lipoproteomics: using mass spectrometry-based proteomics to explore the assembly, structure, and function of lipoproteins.
Hoofnagle A N and Heinecke J W
Journal of Lipid Research, 50(10), 1967-1975 (2009)
Serum apoB levels independently predict the development of non-alcoholic fatty liver disease: A 7-year prospective study.
Wang J, et al.
Liver International : Official Journal of the International Association For the Study of the Liver, 37(8), 1202-1208 (2017)
Alejandro Escobedo et al.
Nutrients, 13(11) (2021-11-28)
Snack alternatives based on common beans (Phaseolus vulgaris L.) have been developed to promote pulse consumption. The purpose of this study was to evaluate the chemical composition, sensory acceptance and the effect of common bean baked snack (CBBS) consumption on
Forty-three loci associated with plasma lipoprotein size, concentration, and cholesterol content in genome-wide analysis.
Chasman DI, et al.
PLoS Genetics, 5(11), e1000730-e1000730 (2009)

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