R4154

Anti-RbAp46, N-terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-RBBP7, Anti-Retinoblastoma-binding Protein 7
• MDL-Nummer: MFCD08705514
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 46 kDa
• Speziesreaktivität: human
• Methode(n): indirect immunofluorescence: 1:200-1:400 using human HeLa cells fixed with paraformaldehyde/Triton; western blot: 1:1,000-1:2,000 μg/mL using nuclear extracts of human HeLa cells
• UniProt-Hinterlegungsnummer: Q16576
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RBBP7(5931)

Beschreibung

Allgemeine Beschreibung

Retinoblastoma-associated binding protein 46 (RbAp46), also referred to as retinoblastoma-binding protein 7 (RBBP7) has 89% amino acid identity with RbAp48. This nuclear protein belongs to the WD-repeat protein family. It has four repeats that ends with Trp-Asp(WD) residues. RbAp46 is an important constituent of the histone modifying and remodeling complexes. RbAp46 gene is located on human chromosome Xp22.13

Spezifität

Anti-RbAp46, N-terminal specifically recognizes human RbAp46.

Immunogen

synthetic peptide corresponding to amino acids 1-14 of human RbAp46, conjugated to KLH via a C-terminal added cysteine residue. The sequence differs in mice by one amino acid.

Anwendung

Anti-RbAp46, N-terminal antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem./physiol. Wirkung

Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in the modification and remodeling of chromatin during cell growth and differentiation. It is associated with the nucleosomal remodeling and deacetylation complex (NURD). It may also play a key role in histone deacetylation and transcription repression.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)