R2655

Monoclonal Anti-Rabbit IgG, Native in Maus hergestellte Antikörper

clone RabT-50, purified from hybridoma cell culture

• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: secondary antibodies
• Klon: RabT-50, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: rabbit
• Darf nicht reagieren mit: human, rat, chicken, cat, bovine, monkey, goat, pig, horse
• Konzentration: ~1 mg/mL
• Methode(n): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Anti-Rabbit IgG, Native antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma RabT-50 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Immunoglobulin G (IgG) is found abundantly in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fragment antigen binding (Fab) arm. The antigen binds to the V regions of the antibody.

This antibody reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.

Spezifität

Anti-Rabbit IgG, Native antibody, Mouse monoclonal reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. It does not recognize human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs.

Immunogen

Enriched fraction of rabbit IgG light chain.

Anwendung

Anti-Rabbit IgG, Native antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunocytochemistry
• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)

Biochem./physiol. Wirkung

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter