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R0503

Sigma-Aldrich

Reaktivrot 120−Agarose

saline suspension, Type 3000-CL

Synonym(e):

Reactive Red Agarose, Red 120-Agarose, Red Agarose

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About This Item

MDL-Nummer:
MFCD00165711
UNSPSC-Code:
23151817
NACRES:
NA.56

Biologische Quelle

plant

Typ

Type 3000-CL

Form

saline suspension

Kennzeichnungsgrad

≥3 μmol per mL

Methode(n)

affinity chromatography: suitable

Matrix

cross-linked 4% beaded agarose

Eignung

suitable for chromatography

Lagertemp.

2-8°C

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Anwendung

Reactive Red 120-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Red 120-agarose has been used to study wheat quality breeding as well as to provide strong evidence that purified human P-glycoprotein (Pgp) functions as an ATP-dependent drug transporter.

Physikalische Form

Suspension in 0.5 M NaCl, enthält 0.02% Thiomersal

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Cibacron Blue 3G-A Dye content ≥55 %

Sigma-Aldrich

B1064

Cibacron Blue 3G-A

C F Barroga et al.
Protein science : a publication of the Protein Society, 5(6), 1093-1099 (1996-06-01)
The nucleotide-binding component of the high-affinity ribose transport system of Escherichia coli, RbsA, was overproduced from a T7-7 expression vector, and the protein was purified. Biochemical analyses of the purified protein indicated that the ATP analogues, 5'-FSBA and 8-azido ATP
S Takeda et al.
Journal of biochemistry, 114(5), 684-690 (1993-11-01)
A particulate fraction consisting of heavy organelles such as nuclei and mitochondria was prepared from Ehrlich ascites tumor cells. From this fraction we have purified a GTP-binding protein with a molecular mass of 33 kDa (MTG33) by guanidine hydrochloride extraction
Galit Yehezkel et al.
The Journal of biological chemistry, 281(9), 5938-5946 (2005-12-16)
In this study, we addressed the presence and location of nucleotide-binding sites in the voltage-dependent anion channel (VDAC). VDAC bound to reactive red 120-agarose, from which it was eluted by ATP, less effectively by ADP and AMP, but not by
S V Ambudkar et al.
Methods in enzymology, 292, 492-504 (1998-08-26)
Human Pgp from the vinblastine-resistant cell line, KB-V1, can be purified by sequential conventional chromatography on DEAE-sepharose CL-6B resin followed by a wheat germ agglutinin column. By including glycerol (osmolyte protectant) and lipid during the solubilization and chromatography procedures most
Kazuo Yamasaki et al.
The Journal of biological chemistry, 277(16), 13615-13619 (2002-02-07)
Conditions were developed in the absence of Ca(2+) for purification, delipidation, and long term stabilization of octaethylene glycol monododecyl ether (C(12)E(8))-solubilized sarcoplasmic reticulum Ca(2+)-ATPase with tightly bound Mg(2+) and F(-), an analog for the phosphoenzyme intermediate without bound Ca(2+). The
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