R0406

Anti-RUNX1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Acute myeloid leukemia 1 protein, AML-1, Anti-CBF-α2, Anti-Core-binding Factor α2 subunit, Anti-Oncogne AML-1, Anti-PEBP2-αB, PEA2-αB, Anti-Polyomavirus enhancer-binding protein 2αB subunit, Anti-Runt-related transcription factor 1, Anti-SL3-3 enhancer factor 2αB subunit, Anti-SL3/AKV core-binding factor αB subunit
• MDL-Nummer: MFCD07370485
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~48 kDa
• Speziesreaktivität: human, mouse, rat (predicted)
• Konzentration: ~1.0 mg/mL
• Methode(n): indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed HeLa cells; western blot: 0.25-0.5 μg/mL using HeLa cell lysate
• UniProt-Hinterlegungsnummer: Q01196
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RUNX1(861) ; mouse ... Runx1(12394) ; rat ... Runx1(50662)

Beschreibung

Allgemeine Beschreibung

RUNX1 encodes a DNA-binding subunit.

Runt-related transcription factor 1 (RUNX1) is a transcription factor that plays an important role in hematopoiesis, osteogenesis and neurogenesis. It is a member of Runt-related transcription factors (RUNXs). It was first identified as a component of Polyomavirus enhancer binding protein 2 (PEBP2) and Moloney murine leukemia virus enhancer core binding factor (CBF). Three isoforms of the protein have been identified: RUNX1a, RUNX1b and RUNX1c. Structurally, it consists of Runt domain at the N-terminal region.

Immunogen

synthetic peptide corresponding to amino acids 42-52 of human RUNX1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Anwendung

Anti-RUNX1 (N-terminal) antibody produced in rabbit is suitable for indirect immunofluorescence and western blot.

Biochem./physiol. Wirkung

RUNX activity is regulated by extracellular signaling pathways resulting in post-translational modifications, such as phosphorylation, acetylation and ubiquitination. It is linked to acute leukemia.

RUNX1 (Runt-related transcription factor 1) plays a vital role in tumour suppression and oncogenic activities. During hematopoiesis, it is involved in hematopoietic development, hematopoietic stem cell homeostasis, and various blood malignancies. It may have clinicopathological impact on the proliferation of human bone marrow cells used for transplantation therapy.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)