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PLED35

Sigma-Aldrich

GenElute Endotoxin-free Plasmid Midiprep Kit

greener alternative

sufficient for 35 purifications

Synonym(e):

GenElute Endotoxin-free Plasmid Kits, endo free midiprep plasmid isolation kit, endotoxin free plasmid extraction kit, endotoxin free plasmid isolation kit, endotoxin free plasmid purification kit, midiprep plasmid isolation kit, plasmid extraction kit, plasmid purification kit, Gen Elute

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About This Item

UNSPSC-Code:
41105501
NACRES:
NA.52

Verwendung

sufficient for 35 purifications

Qualitätsniveau

Grünere Alternativprodukt-Eigenschaften

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Methode(n)

DNA purification: suitable

Grünere Alternativprodukt-Kategorie

Lagertemp.

15-25°C

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Allgemeine Beschreibung

The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.

Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Anwendung

The kit has been used for purifying plasmid DNA, which has been used for transfection.

Leistungsmerkmale und Vorteile

  • High transfection efficiency and yield
  • Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
  • Fast and simple allowing up to 12 preps in less than 2 hours

Rechtliche Hinweise

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Central nervous system

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Comparison of different signal peptides for protein secretion in nonlytic insect cell system.
Olczak M and Olczak T
Analytical Biochemistry, 359(1), 45-53 (2006)
Interferon gamma activated macrophages kill mycobacteria by nitric oxide induced apoptosis.
Herbst S
PLoS ONE, 6(5), e19105-e19105 (2011)
Karyotypically abnormal human ESCs are sensitive to HDAC inhibitors and show altered regulation of genes linked to cancers and neurological diseases.
Lund RJ
Stem Cell Research, 11(3), 1022-1036 (2013)
Novel cationic solid-lipid nanoparticles as non-viral vectors for gene delivery.
Bondi ML
Journal of Drug Targeting, 15(4), 295-301 (2007)

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