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Merck

P8170

Sigma-Aldrich

Protein G−Peroxidase from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

Synonym(e):

Protein G−HRP from Streptococcus sp.

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Rekombinant

expressed in unspecified host

Qualitätsniveau

Konjugat

peroxidase conjugate

Form

lyophilized powder

Methode(n)

direct ELISA: 1:100,000 using human IgG

Lagertemp.

−20°C

Allgemeine Beschreibung

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.

Spezifität

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

Anwendung

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.

Angaben zur Herstellung

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Die Dokumentenbibliothek aufrufen

Christoph Bachmann et al.
Human molecular genetics, 26(2), 320-332 (2016-12-23)
Centronuclear myopathies are early-onset muscle diseases caused by mutations in several genes including MTM1, DNM2, BIN1, RYR1 and TTN. The most severe and often fatal X-linked form of myotubular myopathy (XLMTM) is caused by mutations in the gene encoding the
Evaluation of a modified Rose Bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of Brucella melitensis infection in sheep
Ferreira AC, et al.
Veterninary Research, 34(3), 297-305 (2003)
Thiol peroxidase is an important component of Streptococcus pneumoniae in oxygenated environments
Hajaj B, et al.
Infection and Immunity, IAI-00126 (2012)
P Nol et al.
Clinical and vaccine immunology : CVI, 16(3), 323-329 (2009-01-09)
Monitoring of the kinetics of production of serum antibodies to multiple mycobacterial antigens can be useful as a diagnostic tool for the detection of Mycobacterium bovis infection as well as for the characterization of disease progression and the efficacy of
Purification and some properties of streptococcal protein G, a novel IgG-binding reagent.
Bjorck L and Kronvall G
Journal of Immunology, 133(2), 969-974 (1984)

Protokolle

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

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