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P1167

Sigma-Aldrich

Hypophysenextrakt Rind

lyophilized powder, suitable for cell culture

Synonym(e):

BPE, PEX

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About This Item

MDL-Nummer:
MFCD00163156
UNSPSC-Code:
12352202
NACRES:
NA.75

Biologische Quelle

bovine

Qualitätsniveau

200

Form

lyophilized powder

Wirksamkeit

15 - 200 μg/mL EC50

Qualität

endotoxin tested

Verpackung

pkg of 5 mg

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

≤50 EU/mg

Farbe

brown

Löslichkeit

0.85% sodium chloride: soluble 1 vial/mL, clear to hazy, faintly yellow to dark yellow-orange

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Pituitary Extract, bovine (PEX) is an extract of bovine pituitary tissue containing growth factors.

Anwendung

Pituitary Extract bovine has been used as a component in the Roswell Park memorial institute 1640 (RPMI-1640) to culture Rb-/- epithelial cells. It has also been used as a component in the Dulbecco′s modified Eagle′s medium (DMEM) for culturing Schwann cells.

Biochem./physiol. Wirkung

Pituitary Extract, bovine (PEX) or BPE is essential for mitotic activity and promotes the growth of several types of mammalian cells. It favors the proliferation of keratocytes culture and bovine knee chondrocytes. BPE helps in the synthesis of collagen and glycosaminoglycans (GAGs) in bovine knee chondrocytes.

Physikalische Form

Lyophilized from sodium phosphate buffer

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Simon W Hayward et al.
Methods in molecular biology (Clifton, N.J.), 218, 17-33 (2003-03-06)
The ability to rescue viable prostate precursor tissue from Rb-/-fetal mice has allowed for the generation of Rb-/-prostate tissue and Rb-/-prostate epithelial cell lines. Herein, we provide a protocol for the rescue of urogenital precursor tissue from mouse embryos harboring
Yung-Chih Kuo et al.
Biotechnology progress, 21(6), 1708-1715 (2005-12-03)
In vitro cultivation of primary bovine knee chondrocytes (BKCs), using bovine pituitary extract (BPE) and porous scaffolds composed of polyglycolide (PGA) and 85/15 poly(lactide-co-glycolide) (PLGA), was investigated. Here, BPE was prepared from fresh bovine pituitaries, and cylindrical PGA/PLGA scaffolds with
J C Willey et al.
Journal of cellular physiology, 124(2), 207-212 (1985-08-01)
The mechanisms of action of extracellular mitogens for normal human bronchial epithelial cells (NHBE) were investigated by observing their effects on selected biochemical pathways when the cells were incubated in serum-free media. We find that (a) epidermal growth factor (EGF)
S L Hammond et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(17), 5435-5439 (1984-09-01)
A serum-free medium with bovine pituitary extract as the only undefined supplement has been developed for long-term culture of human mammary epithelial cells. This medium supports serial subculture of normal cells for 10-20 passages (1:10 splits) without conditioning or special
Jie Liu et al.
Animal reproduction science, 115(1-4), 49-57 (2008-12-26)
Different culture systems were evaluated for their ability to support attachment and proliferation of the somatic cells obtained from ovine semen. Ejaculates (n=14) were collected from eight rams representing three breeds, Dorper, Suffolk and Hampshire. All samples were processed immediately
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