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Merck

N6635

Sigma-Aldrich

Nährstoffmischung F-10 aus Schinken

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

Ham’s F-10

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352207
NACRES:
NA.75

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues.

Anwendung

Nutrient Mixture F-10 Ham has been used:

  • for the in vitro tissue culture from rabbit
  • to culture satellite cells from fish white muscle of trout
  • for culturing neonatal porcine islets (NPIs)

Menge

Formulated to contain 9.8 grams of powder per liter of medium.

Rekonstituierung

Supplement with 1.2 g/L sodium bicarbonate.

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Z Bravo et al.
Reproduction in domestic animals = Zuchthygiene, 53(3), 629-635 (2018-02-24)
The follicular fluid exerts an effect on the sperm capacitation of several species; however, these effects vary according to species, both in the sperm motility and in the subsequent acrosome reaction. In this study, the effect of alpaca follicular fluid
Ensieh Sajadi et al.
Journal of cellular biochemistry, 120(10), 17312-17325 (2019-05-22)
The current study was conducted to assess the relationship between testicular cells in spermatogenesis, through which the production of healthy and mature sperm is essential. However, it seems necessary to obtain more information about the three-dimensional pattern of the testis
Katsusuke Hata et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(2), 498-510 (2017-11-28)
The neural cell adhesion molecule (NCAM) is expressed both presynaptically and postsynaptically during neuromuscular junction formation. Genetic deletion in mice of all three isoforms (180, 140, and 120 kDa), or just the 180 isoform, suggested that different isoforms played distinct
Lu Yue et al.
STAR protocols, 1(3), 100128-100128 (2020-12-31)
Quiescent muscle stem cells, also called satellite cells (SCs), are essential for muscle regeneration. However, quiescent SCs are quickly activated during fluorescence-activated cell sorting (FACS) isolation. Here, we present an optimized protocol to isolate quiescent muscle stem cells from fixative-perfused
Cara E Ellis et al.
BioResearch open access, 4(1), 188-197 (2015-08-27)
Since the development of the Edmonton protocol, islet transplantation is increasingly encouraging as a treatment for type 1 diabetes. Strategies to ameliorate problems with the intraportal site include macroencapsulating the islets in diverse biomaterials. Characterization of these biomaterials is important

Protokolle

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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