MAK318

α−Mannosidase Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC-Code: 12161503
• NACRES: NA.84

Eigenschaften

• Nachweisverfahren: colorimetric
• Relevante Krankheit(en): neurological disorders
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

α-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for determining α-Mannosidase (AMA) activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media.

Prinzip

The α-Mannosidase Activity Assay Kit provides a simple and high-throughput adaptable assay for determining α-Mannosidase activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media. In this assay 4-nitrophenol is cleaved from a synthetic substrate. Nitrophenol becomes intensely colored with the addition of the stop reagent, producing a colorimetric (405 nm) result, directly proportional to the enzyme activity in the sample.
One unit of AMA is the amount of enzyme that will convert 1.0 μmole of 4-Nitrophenyl-α-D-mannopyranoside to 4-Nitrophenol and α-D-Mannose per minute at 25 °C and pH 4.5. The kit has a linear detection range of 1–250 unit/L for a 10 minute reaction in a 96 well format.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable