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Merck

MAK119

Sigma-Aldrich

Acetylcholinesterase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(e):

ACHE Activity Assay, RBC Cholinesterase Activity Assay

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric tests

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

neurological disorders

Lagertemp.

room temp

Angaben zum Gen

human ... ACHE(43)
mouse ... ACHE(11423)

Allgemeine Beschreibung

Acetylcholinesterases (AChEs) are enzymes that hydrolyze the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE is located at the synaptic cleft and functions to terminate synaptic transmission by catalyzing the breakdown of ACh allowing cholinergic neurons to return to a resting state after activation. It is also found in membranes of red blood cells, motor and sensory fibers, muscles, nerves and central and peripheral tissues. Changes in AChE activity may result from exposure to certain insecticides, which act as cholinesterase inhibitors. Inhibitors of AChE are also used to treat certain conditions such as dementia.

Anwendung

Acetylcholinesterase activity assay kit has been used to determine the activity of acetylcholinesterase in a rat organophosphate model and in brain tissue homogenates.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Eignung

Suitable for the detection of acetylcholinesterase activity in biological samples such as blood, serum, and plasma.

Prinzip

This assay is an optimized version of the Ellman method in which thiocholine, produced by AChE, reacts with 5,5′-dithiobis(2-nitrobenzoic acid) to form an colorimetric (412 nm) product, proportional to the AChE activity present. One unit of AChE is the amount of enzyme that catalyzes the production of 1.0 μmole of thiocholine per minute at room temperature at pH 7.5. This kit has a linear range of 10-600 units/L of AChE activity.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3


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Abdullah M Alzahrani
Environmental science and pollution research international, 26(16), 16548-16555 (2019-04-14)
Despite the widespread use of the insecticide imidacloprid (IMI), a neonicotinoid, there is an urgent need for documenting information related to its acute toxicity. Therefore, this study aims to explore the markers of IMI acute toxicity in the testes of
Study on social isolation as a risk factor in development of Alzheimer?s disease in rats.
Ali A A, et al.
Brain disorders & therapy., 6(230), 2-2 (2017)
Neuroprotective Effects of AEOL10150 in a Rat Organophosphate Model.
Liang L P, et al.
Toxicological Sciences, 162(2), 611-621 (2017)
Zhen-Xing Zuo et al.
Neural plasticity, 2015, 453170-453170 (2015-12-24)
Chronic pain is a major health issue and most patients suffer from spontaneous pain. Previous studies suggest that Huperzine A (Hup A), an alkaloid isolated from the Chinese herb Huperzia serrata, is a potent analgesic with few side effects. However
Marine Droguerre et al.
Frontiers in neuroscience, 14, 563-563 (2020-07-03)
Donepezil (DPZ) is an acetylcholinesterase inhibitor used in Alzheimer's disease to restore cognitive functions but is endowed with limited efficacy. Recent studies pointed out the implication of astroglial networks in cognitive processes, notably via astrocyte connexins (Cxs), proteins involved in

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