Direkt zum Inhalt
Merck
Alle Fotos(2)

Key Documents

Weitere Dokumente

MAK061

Sigma-Aldrich

Isocitrate Assay Kit

sufficient for 100 colorimetric tests

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)

About This Item

UNSPSC-Code:
12352200
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric tests

Nachweisverfahren

colorimetric

Lagertemp.

−20°C

Allgemeine Beschreibung

The new Isocitrate Assay Kit, MAK524, is now available! Isocitrate is a TCA cycle intermediate that is formed from citrate by the activity of the enzyme aconitase. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. In plants and lower organisms, isocitrate is the branch point from which the glyoxylate shunt operates. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. The Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples.

Eignung

Suitable for quantifying isocitrate in a variety of samples including tissue, cells, food and beverages

Prinzip

Isocitrate concentration is determined by a coupled enzyme assay, which results in a colorometric (450 nm) product, proportional to the isocitrate present. Typical sensitivities of detection for this kit are 1-20 nmoles (0.2 - 5 μg) of isocitrate.

Anwendung

Produkt-Nr.
Beschreibung
Preisangaben

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazardCorrosion
GHS08,GHS05

Signalwort

Danger

H-Sätze

H314,H334

Gefahreneinstufungen

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Artikel

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

Protokolle

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Chromatograms

application for HPLC

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.