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MAC4LRI

Sigma-Aldrich

MetaPolyzyme with RNase inhibitor

Lytic enzyme mixture for RNA extraction

Synonym(e):

Multilytic Enzyme Mix suitable for RNA isolation

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About This Item

UNSPSC-Code:
12352204

Qualitätsniveau

Form

lyophilized powder

Methode(n)

DNA extraction: suitable

Eignung

suitable for microbiology

Anwendung(en)

microbiology

Versandbedingung

wet ice

Lagertemp.

-10 to -25°C

Allgemeine Beschreibung

A common difficulty among microbiome scientists is to extract nucleic acids from difficult to lyse microbes that form capsules or highly resistant spores. Some microbes are difficult to disrupt because the cell walls may form capsules or resistant spores. To address this challenge, protocols that integrate lysing enzymes can be incorporated into the nucleic acid extraction workflow.

Anwendung

Isolating RNA from microbiome samples poses many challenges. RNA molecules are highly labile and are prone to degradation by contaminating RNases. As such, to adapt the MetaPolyzyme for RNA isolation, a broad range RNase inhibitor has been incorporated in order to help prevent RNA degradation by various RNases. MAC4LRI, includes all 6 enzymes from MetaPolyzyme to ensure maximal lysis of microorganisms while protecting the sample RNA from degradation. While it is not possible to inhibit all RNases from a specific microbial environment, we haver incorporated a proprietary RNase Inhibitor to help inhibit as many RNases as possible.


The MetaPolyzyme product family includes MAC4L, MAC4LDF (DNA-Free), SAEO200 (MycoPolyzme, for yeast and fungal lysis, DNA-Free) and MAC4LRI (RNase Inhibitor) and are based on a multi-lytic enzyme mixture formulated for effective lysis of microbiome samples from any sample type. Originally, MetaPolyZyme was developed by Scott Tighe for use on samples from extreme environments and general applications to increase DNA extraction efficiency for any method. To date, these products have been further evaluated and continuously developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG; formerly the Metagenomics Research Group, MGRG). Several publications have cited use of MetaPolyzyme, suggesting various advantages to the usage of MetaPolyzyme for enhancing bacterial lysis.

Leistungsmerkmale und Vorteile

  • Disrupts difficult to lyse microbe samples
  • Protects RNA from degradation

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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