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M7403

Sigma-Aldrich

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

Molecular, Cellular, and Development Biology 153 medium

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Qualitätsniveau

500

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

Versandbedingung

ambient

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

Anwendung

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

Menge

Formulated to contain 17.6 grams of powder per liter of medium.

Rekonstituierung

Supplement with 1.18 g/L sodium bicarbonate.

Ergänzung

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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