M4892
McCoy 5A-Medium
Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Arzneimittelherstellung
Synonym(e):
5A basal medium
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About This Item
Empfohlene Produkte
Qualitätsniveau
Form
powder
Methode(n)
cell culture | mammalian: suitable
Komponenten
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
Versandbedingung
ambient
Lagertemp.
2-8°C
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Allgemeine Beschreibung
McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.
Anwendung
McCoy′s 5A Medium has been used for culturing:
- human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
- human colorectal HCT116 cells to study miR-224 over-expression in cancer
- osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
Biochem./physiol. Wirkung
McCoy′s 5A-Medium wurde ursprünglich zur Unterstützung von Lebertumorzellen durch die Modifikation der in BME vorkommenden Aminosäuren entwickelt. Diese Formulierung wird auch zur Unterstützung des Wachstums in Primärkulturen von Knochenmark, Haut, Gingiva, Niere, Omentum, Nebenniere, Lunge, Milz, Rattenembryos und anderen Zelltypen verwendet.
Menge
Formulated to contain 11.9 grams of powder per liter of medium.
Rekonstituierung
Supplement with 2.2 g/L sodium bicarbonate.
Ergänzung
auch häufig zusammen mit diesem Produkt gekauft
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
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Protokolle
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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