M3567

Anti-Myosin Iβ (Nuclear) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Myh 2, Anti-Myo1c, Anti-Myr 2
• MDL-Nummer: MFCD05665241
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~120 kDa
• Speziesreaktivität: human, mouse, canine, rat
• Methode(n): indirect immunofluorescence: 1:50 using mouse NIH3T3 cells; microarray: suitable; western blot: 1:1,000 using extract of human HeLa cells nuclei and dog MDCK cells
• UniProt-Hinterlegungsnummer: O00159
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MYO1C(4641) ; mouse ... Myo1c(17913) ; rat ... Myo1c(65261)

Beschreibung

Allgemeine Beschreibung

Myosins belong to a superfamily of actin-based motor proteins comprising to date at least 15 classes. Myosin I (also designated myosin-1c, myo1c, M1C, myr 2 and myh 2) is a widely distributed single-headed myosin composed of an N-terminal motor domain, a calmodulin-light chain binding neck region, and a short C-terminal domain. In mammalian cells, myosin I is usually found in the cytoplasm and is especially enriched in perinuclear regions and dynamic cell margins. Myosin Iβ is found in a variety of cells such as kidney tubular cells and inner-ear hair cells.

Spezifität

Recognizes the heavy chain of the nuclear form of human myosin Iβ. Additional bands may be detected in some preparations.

Immunogen

synthetic peptide corresponding to amino acid residues 1-16 of the heavy chain of mouse myosin Iβ (nuclear form). This sequence is missing from the other myosin I isoforms as well as from other currently known myosins.

Anwendung

Anti-Myosin Iβ (Nuclear) antibody produced in rabbit has been used in:

• western blot
• confocal immunofluorescence
• immunogold labeling
• immunofluorescence
• co-immunoprecipitation experiments
• immunostaining

Biochem./physiol. Wirkung

Myosin I is involved in cell motility, microvilli anchorage, vesicular and organelle transport and signal transduction. Myosin I associate with lipid membranes through its tail domain while its motor domain interacts with actin filaments. Myosin Iβ (nuclear) co-localizes with RNA polymerases I and II and possibly acts as a molecular motor to power transcription.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable