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L7634

Sigma-Aldrich

α-Laktose-Agarose

saline suspension

Synonym(e):

alpha-lactose resin

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About This Item

MDL-Nummer:
MFCD00162027
UNSPSC-Code:
41106500
NACRES:
NA.56

Biologische Quelle

bovine milk

Form

saline suspension

Methode(n)

affinity chromatography: suitable

Matrix

6% beaded agarose

Matrixaktivierung

divinyl sulfone

Matrixanbindung

hydroxyl

Matrix-Spacer

5 atoms

Kapazität

6-25 mg/mL binding capacity (lectin from Arachis hypogaea)

Eignung

suitable for chromatography

Lagertemp.

2-8°C

Anwendung

α-Lactose-agarose has been used:
  • in protein chromatography to purify galectins from lysed bacteria cells
  • to capture ricin based on lectin-carbohydrate affinity
  • to study microneme proteins and host-parasite cell surface receptors

α-lLactose-agarose is used in protein chromatography, affinity chromatography and carbohydrate matrices. α--Lactose-agarose has been used to study microneme proteins and host-parasite cell surface receptors.

Physikalische Form

Suspension in 0.15 M NaCl, 0.01 M Natriumphosphat, pH 6.8, enthält 0.02% Natriumazid

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Sepharose™ 4B 45-165 μm bead diameter

Sigma-Aldrich

4B200

Sepharose™ 4B

Lactose tested according to Ph. Eur.

Sigma-Aldrich

17814

Lactose

Cyanogenbromid-aktivierte Sepharose™ 4B lyophilized powder

Sigma-Aldrich

C9142

Cyanogenbromid-aktivierte Sepharose™ 4B

Lactose, wasserfrei European Pharmacopoeia (EP) Reference Standard

A1206000

Lactose, wasserfrei

Divinylsulfon contains hydroquinone as inhibitor, ≥96%

Sigma-Aldrich

V3700

Divinylsulfon

C Allison Gray et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(21), 7982-7987 (2004-05-19)
Secretions of the uterus support survival and growth of the conceptus (embryo/fetus and associated membranes) during pregnancy. Galectin-15, also known as OVGAL11 and a previously uncharacterized member of the galectin family of secreted beta-galactoside lectins containing a conserved carbohydrate recognition
Christophe Dussouy et al.
Chemical biology & drug design, 96(4), 1123-1133 (2020-03-29)
Galectins play key roles in numerous biological processes. Their mode of action depends on their localization which can be extracellular, cytoplasmic, or nuclear and is partly mediated through interactions with β-galactose containing glycans. Galectins have emerged as novel therapeutic targets
E V Lourenço et al.
Glycobiology, 11(7), 541-547 (2001-07-12)
Host cell invasion by Toxoplasma gondii is a multistep process with one of the first steps being the apical release of micronemal proteins that interact with host receptors. We demonstrate here that micronemal protein 1 (MIC1) is a lactose-binding lectin.
Nadine Keller et al.
Infection and immunity, 72(8), 4791-4800 (2004-07-24)
Microneme proteins have been shown to play an important role in the early phase of host cell adhesion, by mediating the contact between the parasite and host cell surface receptors. In this study we have identified and characterized a lectin-like
Na Yang et al.
Journal of molecular biology, 387(3), 694-705 (2009-04-14)
Lectin AAL (Agrocybe aegerita lectin) from the edible mushroom A. aegerita is an antitumor protein that exerts its tumor-suppressing function via apoptosis-inducing activity in cancer cells. The crystal structures of ligand-free AAL and its complex with lactose have been determined.
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