L7261

Lipopolysaccharide aus Salmonella typhimurium

purified by trichloroacetic acid extraction

• Synonym(e): LPS
• MDL-Nummer: MFCD00164404
• UNSPSC-Code: 12352201
• NACRES: NA.25

Eigenschaften

• Biologische Quelle: Salmonella enterica (Serotype typhimurium)
• Qualitätsniveau: 200
• Form: lyophilized powder
• Aufgereinigt durch: trichloroacetic acid extraction
• Verunreinigungen: 1-10% Protein (Lowry)
• Farbe: white to faint yellow
• Löslichkeit: water: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

This product is TCA extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS serotype has been used to induce NOS in rats and guinea pigs.

Anwendung

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)