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Wichtige Dokumente
L6037
Escort™ II Transfection Reagent
Lipid formulation for nucleic acid transfections
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About This Item
UNSPSC-Code:
12352200
Qualität
for molecular biology
Form
dried film
Verwendung
1.5 mL sufficient for 150 transfections
Lagertemp.
2-8°C
Allgemeine Beschreibung
Escort™ II is a unique formulation of a proprietary cationic lipid and the neutral lipid DOPE. This liposome-forming compound is used for transfection of nucleic acids into eukaryotic cells.
Anwendung
Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 25 μl Escort™ II and 5 μg DNA (in provided unique DNA Dilution buffer) per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ II:
B16-F0
BHK-21
CHO-K1
COS-1
COS-7
CV-1
D. mel (S2)
F9
HepG2
HEK293
HeLa
HUVEC
K562
L929
MDCK
NIH3T3
P19
PC-12
Vero
B16-F0
BHK-21
CHO-K1
COS-1
COS-7
CV-1
D. mel (S2)
F9
HepG2
HEK293
HeLa
HUVEC
K562
L929
MDCK
NIH3T3
P19
PC-12
Vero
Leistungsmerkmale und Vorteile
- Suitable for stable and transient transfection
- High efficiency for a wide variety of cell types
- Compatible with both serum and serum-free transfection protocols
- Low toxicity
Komponenten
Escort™ II Transfection Kit (0.75 mL) contains:
0.75 mL Escort II Reagent (L6037)
0.8 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Escort™ II Transfection Kit (1.5 mL) contains:
1.5 mL Escort II Reagent (L6037)
1.6 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Note the formulation of Escort™ II is confidential.
0.75 mL Escort II Reagent (L6037)
0.8 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Escort™ II Transfection Kit (1.5 mL) contains:
1.5 mL Escort II Reagent (L6037)
1.6 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Note the formulation of Escort™ II is confidential.
Vorsicht
Do not freeze.
Prinzip
A stable complex is formed when Escort™ II is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.
Rechtliche Hinweise
Escort is a trademark of Sigma-Aldrich Co. LLC
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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