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Merck

L4268

Sigma-Aldrich

Lipopolysaccharide aus Klebsiella pneumoniae

purified by phenol extraction

Synonym(e):

LPS

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352201
NACRES:
NA.25

Biologische Quelle

bacterial (Klebsiella pneumoniae)

Qualitätsniveau

Form

lyophilized powder

Aufgereinigt durch

phenol extraction

Verunreinigungen

<3% Protein (Lowry)

Farbe

white to faint yellow

Löslichkeit

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

This product is phenol extracted from Klebsiella pneumoniae. The source strain is ATCC 15380.

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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Teresa García-Lezana et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37(3), 927-937 (2016-05-08)
Hepatic encephalopathy has traditionally been considered a reversible disorder. However, recent studies suggested that repeated episodes of hepatic encephalopathy cause persistent impairment leading to neuronal loss. The aims of our study were the development of a new animal model that
Jinling Zhang et al.
Biosensors & bioelectronics, 99, 312-317 (2017-08-09)
We report a smartphone label-free biosensor platform based on grating-coupled surface plasmon resonance (GC-SPR). The sensor system relies on the smartphone's built-in flash light source and camera, a disposable sensor chip with Au diffraction grating and a compact disk (CD)
Nora S Wolff et al.
Intensive care medicine experimental, 8(1), 47-47 (2020-08-26)
The microbiome has emerged as an important player in the pathophysiology of a whole spectrum of diseases that affect the critically ill. We hypothesized that differences in microbiota composition across vendors can influence murine models of pulmonary lipopolysaccharide (LPS) inflammation
Christian Maueröder et al.
Frontiers in immunology, 7, 583-583 (2016-12-27)
In this study, we identified and characterized the potential of a high ratio of bicarbonate to CO2 and a moderately alkaline pH to render neutrophils prone to undergo neutrophil extracellular trap (NET) formation. Both experimental settings increased the rate of
C Arnold Spek et al.
Cells, 10(9) (2021-09-29)
CCAAT/enhancer-binding protein delta (C/EBPδ) is a member of the C/EBP family of transcription factors. According to the current paradigm, C/EBPδ potentiates cytokine production and modulates macrophage function thereby enhancing the inflammatory response. Remarkably, however, C/EBPδ deficiency does not consistently lead

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