L1882

Lektin aus Triticum vulgaris (Weizen)

agarose conjugate, saline suspension

• Synonym(e): Weizenkeim-Agglutinin
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Konjugat: agarose conjugate
• Qualitätsniveau: 200
• Form: saline suspension
• Kennzeichnungsgrad: 5-10 mg per mL
• Matrix: cross-linked 4% beaded agarose
• Matrixaktivierung: cyanogen bromide
• Kapazität: ≥0.1 mg/mL binding capacity (ovomucoid)
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Anwendung

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Biochem./physiol. Wirkung

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

WGA ist nicht Blutgruppen-spezifisch, hat aber eine Affinität zu N-Acetyl-β-D-Glucosaminylresten und N-Acetyl-β-D-Glucosamin-Oligomeren. WGA enthält keine proteingebundenen Kohlenhydrate.

Physikalische Form

Suspension in 1,0 M NaCl mit 15 mM Natriumazid

Angaben zur Herstellung

Konjugate werden aus affinitätsgereinigtem Lektin hergestellt.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Gloves