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K4018

Sigma-Aldrich

PKCζ, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Rekombinant

expressed in baculovirus infected Sf9 cells

Qualitätsniveau

200

Produktlinie

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

Form

buffered aqueous glycerol solution

Spezifische Aktivität

97-131 nmol/min·mg

Mol-Gew.

~93 kDa

UniProt-Hinterlegungsnummer

Q05513

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... PRKCZ(5590)

Biochem./physiol. Wirkung

PKCζ (protein kinase C ζ) is a atypical PKC and works independent of Ca2+ and diacylglycerol. It is involved in MAPK (mitogen-activated protein kinase)-induced nuclear factor κ B activation. It also stabilizes DNA mismatch-repair proteins, participates in laeukemic cell differentiation, cell migration and chemotaxis. PKCζ have tumour suppressor as well as oncogenic properties. Upregulation of PKCζ causes apoptosis in ovarian carcinoma cells. On the other hand, absence of PKCζ decreases tumorigenic activity in a colon cancer mouse model.

Physikalische Form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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S J Kim et al.
Biochemical and biophysical research communications, 237(2), 336-339 (1997-08-18)
To examine whether multiple protein kinase C (PKC) isoforms could interact or crosstalk, phorbol ester-insensitive atypical PKC (aPKC) zeta isoform was overexpressed in NIH3T3 cells, and the cells were stimulated with phorbol ester to activate diacylglycerol-dependent conventional (cPKC) and novel
A Romanelli et al.
Molecular and cellular biology, 19(4), 2921-2928 (1999-03-19)
p70 S6 kinase (p70S6K) is an important regulator of cell proliferation. Its activation by growth factor requires phosphorylation by various inputs on multiple sites. Data accumulated thus far support a model whereby p70S6K activation requires sequential phosphorylations at proline-directed residues
S A Hartsink-Segers et al.
Leukemia, 29(2), 304-311 (2014-07-06)
Both tumour suppressor and oncogenic functions have been ascribed to the atypical zeta isoform of protein kinase C (PKCζ), whereas its constitutively active form PKMζ is almost exclusively expressed in the brain where it has a role in long-term memory.

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