I8648

Interleukin-15 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): IL-15
• MDL-Nummer: MFCD00675504
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.2-4.0 ng/mL EC₅₀
• Mol-Gew.: antigen predicted mol wt ~12.5 kDa
• Verpackung: pkg of 5 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P40933
• Lagertemp.: −20°C
• Angaben zum Gen: human ... IL15(3600)

Beschreibung

Biochem./physiol. Wirkung

IL-15 (interleukin 15) is a proinflammatory pleiotropic cytokine and is a connection between innate and adaptive immune responses. It participates in the proliferation, survival and activation of various lymphocytes lineages. IL-15 works by associating with a high affinity heterotrimeric receptor complex containing IL-15Rα, IL-2/15Rβ and IL-Rγ. It is present in different forms, the soluble form, the hyper IL-15 formed by association between IL-15 receptor α chain and soluble cytokine, and the membrane bound isoform.

Interleukin-15 was first isolated from the supernatant of a cultured simian kidney epithelial cell line. The cDNA encodes a 162 amino acid precursor protein with a 48 amino acid signal peptide that is cleaved to yield a 114 amino acid mature protein. Human IL-15 shares sequence identity with simian (~97%) and murine (~73%). IL-15 competes for binding sites with IL-2, as both IL-2 and IL-15 stimulate the growth of cells through the IL-2 receptor.

Physikalische Form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline, pH 7.4 with 50 μg bovine serum albumin per 1 μg as a carrier protein.

Hinweis zur Analyse

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)