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I3911

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, lyophilized powder, for the inhibition of serine, cysteine, aspartic and metalloproteases, for general use, lyophilized powder

Synonym(e):

protease inhibitor

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, Animal Component Free, for general use, lyophilized powder

Qualitätsniveau

200

Form

lyophilized powder

Löslichkeit

water: soluble

Lagertemp.

−20°C

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Allgemeine Beschreibung

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid-proteases, and aminopeptidases.

Spezifität

Inhibits serine, cysteine, aspartic, and metalloproteases.

Anwendung

Animal component free (ACF). Optimized and tested for general use. Protease Inhibitor Cocktail, Animal Component Free has been used to prevent protein lysis in saliva samples, peritoneal residential macrophage cells and in embryo culture medium.

Komponenten

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Menge

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Physikalische Form

Lyophilized powder that is water soluble.
The components of this ACF cocktail do not originate from animal sources and can thus be used in the production of animal free proteins.

Piktogramme

Corrosion
GHS05

Signalwort

Danger

H-Sätze

H314

Gefahreneinstufungen

Eye Dam. 1 - Skin Corr. 1A

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
Lopucki M, et al.
Molecular Medicine Reports, 17(6), 8253-8259 (2018)
Functional evaluation of multiple human transgenes to overcome immunological and coagulation barriers associated with pig-to-human xenotransplantation.
Bongoni, A.K. et al.
Thesis, 108-108 (2013)
Marija Holcar et al.
Scientific reports, 10(1), 21346-21346 (2020-12-09)
Human plasma is a complex fluid, increasingly used for extracellular vesicle (EV) biomarker studies. Our aim was to find a simple EV-enrichment method for reliable quantification of EVs in plasma to be used as biomarker of disease. Plasma of ten
Early protein profile of human embryonic secretome.
Foresta C, et al.
Frontiers in Bioscience, 21, 620-634 (2016)
Carlo Foresta et al.
Frontiers in bioscience (Landmark edition), 21, 620-634 (2015-12-29)
Embryos obtained by in vitro fertilization are currently assessed by morphology, but displays limitations with over 70% of embryos failing to implant. In this study, we performed HPLC-MS/MS analysis on the conditioned medium obtained from 50 human embryos at the
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