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Merck

HPA002689

Sigma-Aldrich

Anti-WASHC1 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(e):

Anti-FAM39E, Anti-FLJ00038, Anti-WASH1

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About This Item

UNSPSC-Code:
12352203
Human Protein Atlas-Nummer:
NACRES:
NA.43

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Produktlinie

Prestige Antibodies® Powered by Atlas Antibodies

Form

buffered aqueous glycerol solution

Speziesreaktivität

human

Erweiterte Validierung

RNAi knockdown
Learn more about Antibody Enhanced Validation

Methode(n)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:1000-1:2500

Immunogene Sequenz

GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Allgemeine Beschreibung

WASH complex subunit 1 (WASHC1) is located on human chromosome 9p24.3. It belongs to Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) family. WASHC1 protein has an actin binding domain.

Immunogen

WASH complex subunit 1 recombinant protein epitope signature tag (PrEST)

Anwendung

Anti-WASHC1 antibody produced in rabbit has been used:
  • in the detection of WASH complex in Dictyostelium cells by immunofluorescence imaging.
  • in the detection of WASH proteins in HeLa cells using confocal microscopy.
  • in western blotting for the detection of WASH1 complexes in human leukemic Jurkat T cells.

Biochem./physiol. Wirkung

WASH exists as multiple complex protein in HeLa and bovine cells. WASH proteins coordinate the migration of endosomal α5β1 integrin. WASHC1 protein regulates actin cytoskeleton organisation. WASH1 protein is regarded as actin-nucleating promoting factor. WASH protein complex interacts with actin related protein, Arp2/3 and mediates actin networking.

Leistungsmerkmale und Vorteile

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Verlinkung

Corresponding Antigen APREST74318

Physikalische Form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Rechtliche Hinweise

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

The Arp2/3 activator WASH regulates alpha5beta1-integrin-mediated invasive migration
Zech T, et al.
Journal of Cell Science, 124(22), 3753-3759 (2011)
Functional characterization of Wiskott Aldrich Syndrome protein and scar homolog (WASH), a bi-modular nucleation promoting factor (NPF) able to interact with biogenesis of lysosome related organelle subunit 2 (BLOS-2) and gamma-tubulin
Monfregola J, et al.
The Journal of Biological Chemistry, jbc-M109 (2010)
Catherine M Buckley et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5906-E5915 (2016-09-21)
Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is
Human subtelomeric WASH genes encode a new subclass of the WASP family
Linardopoulou EV, et al.
PLoS Genetics, 3(12), e237-e237 (2007)
The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics
Harbour ME, et al.
The Journal of Biological Chemistry, jcs-071472 (2010)

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