Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

H8032

Sigma-Aldrich

Bouillon nach Hanahan (SOB-Medium)

powder microbial growth medium

Synonym(e):

SOB Medium

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
41106200
NACRES:
NA.85

Qualität

for molecular biology

Qualitätsniveau

100

Beschreibung

recommended for cultivation of recombinant E. coli.

Sterilität

non-sterile

Form

powder

Zusammensetzung

KCl, 0.186 g/L
MgSO4, 2.4 g/L
NaCl, 0.5 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

Methode(n)

microbiological culture: suitable

pH-Wert

6.8-7.2(2.8% solution)

Anwendung(en)

food and beverages
microbiology

Lagertemp.

room temp

Eignung

nonselective for Escherichia coli
nonselective for coliforms

Verwandte Kategorien

Allgemeine Beschreibung

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.

Anwendung

Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Leistungsmerkmale und Vorteile

Hannahan′s Broth (SOB) powder provides:
  • Standard formulation
  • An inexpensive alternative to liquid

Rekonstituierung

Herstellung
1. 28.09 g in 1 L destillertem Wasser suspendieren.
2. Für 15 Minuten bei 121°C autoklavieren.

Sonstige Hinweise

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Difco Manual, 444-444 (1998)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
Arenas-LC, et al.
Biotechnology for Biofuels, 12, 150-150 (2019)
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth
Francesca Magnani et al.
Nature protocols, 11(8), 1554-1571 (2016-07-29)
The thermostability of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals that are suitable for structure determination. However, many mammalian MPs are too unstable for crystallization. We developed

Protokolle

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.