H7290

Anti-HtrA2 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-High Temperature Requirement protein A2, Anti-HtrA serine peptidase 2, Anti-Omi, Anti-PARK13, Anti-PRSS25, Anti-Protease, Serine, 25
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~36 kDa (also 50 kDa band corresponding to HtrA2 precursor)
• Speziesreaktivität: human, rat
• Verpackung: antibody small pack of 25 μL
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Konzentration: ~1.5 mg/mL
• Methode(n): western blot: 2-4 μg/mL using rat liver microsomal fraction and HEK-293 cells expressing human HTRA2
• UniProt-Hinterlegungsnummer: O43464
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HTRA2(27429) ; rat ... Htra2(297376)

Beschreibung

Allgemeine Beschreibung

High-temperature requirement protein A2 (HtrA2) belongs to the HtrA family of serine proteases. This protein is located in the mitochondria and is made up of N-terminal mitochondrial localization sequence (MLS), an inhibitor of apoptosis protein (IAP) binding domain (IBM), a single C-terminal PDZ domain that mediates protein-protein interaction, and a conserved catalytic domain of serine proteases. The HTRA2 gene is located on the human chromosome at 2p13.1.

Spezifität

Anti-HtrA2 specifically recognizes human and rat HtrA2.

Anwendung

Anti-HtrA2 antibody produced in rabbit may be used in immunoblotting.

Biochem./physiol. Wirkung

High-temperature requirement A2 (HtrA2) exhibits endoproteolytic activity by cleaving misfolded proteins and other cellular proteins. This protein possesses protease activity, which is required for mitochondrial homeostasis. HtrA2 is released from the mitochondria as a mature form in response to apoptotic stimuli and is involved in regulating apoptosis through multiple pathways, including caspase-dependent and caspase-independent cell death. Mature HtrA2 promotes cytochrome c-dependent caspase activation by neutralizing inhibitors of apoptosis proteins (IAPs). Mutations that inactivate HtrA2 are associated with neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)