H5505

Histon aus Kalbsthymus

Type III-S

• CAS-Nummer: 9064-47-5
• Beilstein: 1895465
• MDL-Nummer: MFCD00131329
• UNSPSC-Code: 41106305
• NACRES: NA.51

Eigenschaften

• Biologische Quelle: bovine (calf) thymus
• Typ: Type III-S
• Form: powder
• Konzentration: ≥60% protein (biuret)
• Methode(n): activity assay: suitable
• Löslichkeit: H₂O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Histone is suitable for use:

• as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
• in Cdk5 activity assay
• in histone H1 kinase assay
• as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
• as an acceptor of ³²P from [γ-³²P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

Biochem./physiol. Wirkung

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

Leistungsmerkmale und Vorteile

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Sonstige Hinweise

Characterized as mainly subgroup f₁ by SDS-PAGE

Angaben zur Herstellung

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)