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GERPN3000

ECL™ Direct Nucleic Acid

Cytiva RPN3000, pack of 1 ea

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About This Item

UNSPSC-Code:
41105300
NACRES:
NA.31

Verpackung

pack of 1 ea

Hersteller/Markenname

Cytiva RPN3000

Lagertemp.

2-8°C

Allgemeine Beschreibung

ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.

Anwendung

ECL™ Direct Nucleic Acid has been used in Western blot, southern blot and hybridization.

Leistungsmerkmale und Vorteile

  • Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
  • Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
  • No need to strip blots before reprobing.
  • For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
  • Consistent results combining strong signals with very Low backgrounds.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

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Piktogramme

Exclamation mark
GHS07

Signalwort

Danger

Lagerklassenschlüssel

12 - Non Combustible Liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Kostyantyn V Dmytruk et al.
BMC biotechnology, 11, 58-58 (2011-05-27)
The detection and quantification of uric acid in human physiological fluids is of great importance in the diagnosis and therapy of patients suffering from a range of disorders associated with altered purine metabolism, most notably gout and hyperuricaemia. The fabrication
The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii.
Leao SC, et al.
PLoS ONE, 8(4), e60746-e60746 (2013)
CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae.
Sasano Y and Harashima S
Bio-protocol, 7(10) (2017)
Elevated urinary level of vitamin D binding protein as a novel biomarker for diabetic nephropathy.
Tian XQ, et al.
Experimental and Therapeutic Medicine, 7(2), 411-416 (2014)
Transfer of antibiotic resistance marker genes between lactic acid bacteria in model rumen and plant environments.
Toomey N, et al.
Applied and Environmental Microbiology, 75(10), 3146-3152 (2009)
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