GE28-9670-66
Protein G Mag Sepharose™ Xtra
Cytiva 28-9670-66, pack of 2 × 1 mL
About This Item
Empfohlene Produkte
ligand
protein G
Verpackung
pack of 2 × 1 mL
Hersteller/Markenname
Cytiva 28-9670-66
Lagerbedingungen
(20% Ethanol, 10% medium slurry)
Parameter
Room temperature temp. range
Matrix
paramagnetic, spherical, highly cross-linked agarose particles
Kapazität
27 mg binding capacity(human IgG/ml gel)
Lagertemp.
2-8°C
Allgemeine Beschreibung
Anwendung
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose™ Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose™ medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.
Leistungsmerkmale und Vorteile
- Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
- High purity and yield
- Robust parallel screening of antibodies with high reproducibility
- Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
- Visible and dense Sepharose™ based magnetic beads give increased simplicity for handling
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
Protokolle
This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.
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