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GE28-9031-30

Streptavidin High Performance Spintrap™

Cytiva 28-9031-30, pack of 16 columns

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.25

Leistungsmerkmale

wetted part: no

Verpackung

pack of 16 columns

Hersteller/Markenname

Cytiva 28-9031-30

Lagerbedingungen

(20% Ehtanol)
 (20% Ethanol)

Bettvolumen

800 μL

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning

2-10.5

working range

4-9

Kapazität

>300 nmol/mL binding capacity(biotin/ml medium 6 mg biotinylated BSA/ml medium)
 binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

Lagertemp.

2-8°C

Allgemeine Beschreibung

Streptavidin HP SpinTrap™ are prepacked, single-use spin columns and 96-well filter plates for the preparation of protein samples and enrichment of proteins of interest from clarified cell lysates and biological fluids.

Anwendung

Streptavidin HP SpinTrap™ can be used for Protein enrichment, where a biotinylated antibody (or similar affinity molecule) is attached to the streptavidin and the Protein of interest is enriched through the affinity interaction with the antibody. The columns can also be used for the direct enrichment of Proteins that are biotinylated. By binding a specific biotinylated Protein to the column, Protein-Protein interactions can also be investigated.

Leistungsmerkmale und Vorteile

  • For simple, small-scale Protein enrichment through immobilized biotinylated biomolecules.
  • Fast and flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Useful for exploiting either the strong interaction of biotin and streptavidin or the somewhat weaker interaction of 2-iminobiotin and streptavidin.
  • Each column is packed with Streptavidin Sepharose™ High Performance for reproducibility and high performance.
  • Easy scale-up with HiTrap® Streptavidin HP prepacked columns.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from GE Healthcare.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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