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GE17-0965-10

Phenyl Sepharose™ 6 Fast Flow / Low Sub

Cytiva 17-0965-10, pack of 25 mL

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

ligand

phenyl

Verpackung

pack of 25 mL

Hersteller/Markenname

Cytiva 17-0965-10

Matrix

6% cross-linked agarose

Partikelgröße

45-165 μm

Durchschnittlicher Durchmesser

90 μm

cleaning in place

2-14

working range

3-13

Eignung

suitable for bioprocess medium

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Verwandte Kategorien

Allgemeine Beschreibung

Phenyl Sepharose™ 6 Fast FLow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification requiring low to medium hydrophobicity.

Anwendung

Phenyl Sepharose™ 6 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Phenyl Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.

Phenyl Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Leistungsmerkmale und Vorteile

  • Standard aromatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Designed for initial and intermediate purification requiring Low to medium hydrophobicity
  • Two levels of substitution help find optimal selectivity and binding capacity for a given application
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Q Sepharose™ Fast Flow preswollen, 45-165 μm (wet), exclusion limit ~4,000,000 Da

Sigma-Aldrich

Q1126

Q Sepharose™ Fast Flow

Artikel

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Protokolle

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

Sepharose High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose High Performance and we present some examples of media screening, capture and purification.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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