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GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

Synonym(e):

HiTrap Protein G, Protein G affinity column

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Verpackung

pack of 2 × 1 mL

Hersteller/Markenname

Cytiva 17-0404-03

Lagerbedingungen

(20% Ehtanol)
 (20% Ethanol)

Parameter

42 psi

Bettgröße

7 mm × 25 mm

Bettvolumen

1 mL

Säule ID

7 mm

Größe

Durchschnittliche Teilgröße

34 μm

cleaning

2-9

working range

3-9

Kapazität

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Leistungsmerkmale und Vorteile

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

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Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

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This page shows how to prepare samples for purification with affinity chromatography.

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