G8918

Anti-GRP78/BiP (GL-19) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-78-kDa Glucose-Regulated Protein, Anti-Immunoglobulin Heavy Chain Binding Protein
• MDL-Nummer: MFCD01865530
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 78 kDa
• Speziesreaktivität: mouse, hamster, human
• Erweiterte Validierung: independent; Learn more about Antibody Enhanced Validation
• Methode(n): immunocytochemistry: 1:1,000 using mouse fibroblasts NIH3T3 cells; microarray: suitable; western blot: 1:3,000 using human epitheloid carcinoma HeLa whole cell extract
• UniProt-Hinterlegungsnummer: P11021
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HSPA5(3309) ; mouse ... Hspa5(14828)

Beschreibung

Allgemeine Beschreibung

GRP78/BiP is a Ca²⁺-binding molecular chaperone located in the endoplasmic reticulum (ER). It is a highly conserved protein.

Anwendung

Anti-GRP78/BiP (GL-19) antibody produced in rabbit has been used in:

• western blotting
• immunocytochemistry
• immunohistochemistry

Biochem./physiol. Wirkung

GRP78 (78-kDa glucose-regulated protein) is essential for cell viability. GRP78/BiP is induced by stress-inducing agents or by conditions that adversely affect endoplasmic reticulum(ER) function, including oxidative stress, chemical toxicity, Ca²⁺ ionophores, inhibition of the ER Ca²⁺ -ATPase, inhibitors of glycosylation and hypoxia. GRP78/BiP is critical for maintenance of Ca²⁺ homeostasis and the prevention of apoptosis. GRP78/BiP levels are reduced in Alzheimer′s disease brains and the decreased expression of GRP78/BiP is found associated with mis-sense mutations in presenilin-1 (PS-1).

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable