G8255

Glutam-Brenztraubentransaminase aus Schweineherz

lyophilized powder, ≥75 units/mg protein

• Synonym(e): L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
• CAS-Nummer: 9000-86-6
• EC-Nummer: 2.6.1.2 (BRENDA, IUBMB)
• Beilstein: 3562140
• EG-Nummer: 232-561-4
• MDL-Nummer: MFCD00131192
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Porcine heart
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥75 units/mg protein
• Zusammensetzung: Protein, 75-95%
• Lagerbedingungen: (Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized; persons)
• Methode(n): cell culture | mammalian: suitable
• Farbe: light yellow to yellow
• Fremdaktivität: L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%; glutamic-oxalacetic transaminase ≤0.1%
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Research area: Cell Signaling

Anwendung

Glutamic-Pyruvic Transaminase from porcine heart has been used:

• in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
• to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
• in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies

Biochem./physiol. Wirkung

Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Einheitendefinition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physikalische Form

Lyophilized powder containing citrate and acetate

Hinweis zur Analyse

Protein determined by biuret

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)