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G5893

Sigma-Aldrich

Gamborg′s B-5 Basal Medium with Minimal Organics

fine powder, suitable for plant cell culture

Synonym(e):

B-5 Basal Medium, Basal Medium, Minimal Organics Medium

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.72

Form

fine powder

Qualitätsniveau

200

Methode(n)

cell culture | plant: suitable

Anwendung(en)

agriculture

Lagertemp.

2-8°C

Allgemeine Beschreibung

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Anwendung

Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
  • as a component of liquid media for the tissue culture of Brassica rapa
  • as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
  • as a component of solid GM medium for germination of Arabidopsis seeds

Alternative Formulierung

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation

Menge

Formulated to contain 3.2 grams of powder per liter of medium.

Piktogramme

Flame over circle
GHS03

Signalwort

Warning

H-Sätze

H272

Gefahreneinstufungen

Ox. Sol. 3

Lagerklassenschlüssel

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Experiments in Plant Tissue Culture (1985)
Leigh Mickelson-Young et al.
Journal of experimental botany, 67(21), 6077-6087 (2016-11-05)
The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on
Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA
Andersen S E, et al.
Plant Physiology, 116(1), 299-307 (1998)
RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
Lackey E, et al.
The New phytologist, 186(1), 207-215 (2010)
Martin E Kolewe et al.
Plant cell reports, 29(5), 485-494 (2010-03-11)
Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus
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Artikel

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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