G0282

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): GM-CSF
• MDL-Nummer: MFCD00241918
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Granulocyte-Macrophage Colony-Stimulating Factor from mouse, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤0.2 ng/mL EC₅₀ (corresponds to ≥5 × 10⁶ units/mg)
• Qualität: endotoxin tested
• Mol-Gew.: ~15 kDa (125 amino acids including N-terminal methionine)
• Verpackung: pkg of 5 and 50 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1 EU/mg
• Farbe: white
• Löslichkeit: water: soluble, clear, colorless
• UniProt-Hinterlegungsnummer: P01587
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... CSF2(12981) , Csf2(12981)

Beschreibung

Biochem./physiol. Wirkung

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physikalische Form

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

Hinweis zur Analyse

The EC₅₀ activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)