F4042

Monoclonal ANTI-FLAG^® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-ddddk, Anti-dykddddk
• MDL-Nummer: MFCD00282246
• UNSPSC-Code: 12352203
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: M5, monoclonal
• Form: buffered aqueous solution
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: all
• Konzentration: 2-5 mg/mL
• Methode(n): western blot (chemiluminescent): 10 μg/mL
• Isotyp: IgG1
• Immunogene Sequenz: DYKDDDDK
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Monoclonal ANTI-FLAG^® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Anwendung

Monoclonal ANTI-FLAG^® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.

Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca²⁺-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Physikalische Form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Angaben zur Herstellung

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Sonstige Hinweise

Antibody is not calcium dependent.

Rechtliche Hinweise

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable