F3133

Fibroblasten-Wachstumsfaktor aus Rinderhypophyse

lyophilized, suitable for cell culture

• Synonym(e): FGF
• CAS-Nummer: 62031-54-3
• MDL-Nummer: MFCD00166724
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Fibroblasten-Wachstumsfaktor aus Rinderhypophyse, suitable for cell culture
• Biologische Quelle: bovine pituitary glands
• Qualitätsniveau: 200
• Form: lyophilized powder
• Wirksamkeit: 1-100 ng/mL
• Mol-Gew.: 16.4 kDa
• Verpackung: pkg of 10 and 5X10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Farbe: white to off-white
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Anwendung

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.

Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Biochem./physiol. Wirkung

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

Qualität

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Angaben zur Herstellung

Purified glands by a modification of the method of Gospodarowicz.

Hinweis zur Analyse

The biological activity is measured using a 3-day MTT assay.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)