F2653
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat
flow cytometry grade, affinity isolated antibody, buffered aqueous solution
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About This Item
Empfohlene Produkte
Biologische Quelle
goat
Qualitätsniveau
Konjugat
FITC conjugate
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
secondary antibodies
Qualität
flow cytometry grade
Klon
polyclonal
Form
buffered aqueous solution
Methode(n)
flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
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Allgemeine Beschreibung
IgG, a monomer, is the principal antibody circulating in blood which assist in phagocytic destruction of foreign microorganism and is the only class of Ig that can cross the placenta in humans. Cell-sorting done by differential binding of anti-mouse IgG (Fab Specific) - FITC antibody separates tumour cells from macrophages. This antibody can be used to incubate somatic cell nuclear transfer (SCNT) rat embryos for immunocytochemical observation. It also can be used as a secondary antibody to study the dynamics of activated B lymphocytes in small intestine, Peyer′s patch germinal center as well as non-germinal center, spleen and the mesenteric lymph node. Anti-mouse IgG antibody reacts specifically with all mouse immunoglobulins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Spezifität
Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Immunogen
Purified Fab fragments of mouse IgG.
Anwendung
Anti-Mouse IgG (Fab Specific) FITC Conjugate antibody is used in traditional method of immunocytochemical assessment of ZAP-70 expression. It can also be used in immunocytochemical observation of the SCNT embryos.
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in:
- indirect immunofluorescence staining
- immunofluorescence staining
- flow cytometry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Flow cytometry/Cell sorting (1 paper)
Biochem./physiol. Wirkung
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Sonstige Hinweise
Antibody adsorbed with bovine, equine and human serum proteins.
Physikalische Form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Angaben zur Herstellung
Adsorbed to reduce background staining with bovine, horse or human samples.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Analysenzertifikate (COA)
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Agnieszka Bojarska-Junak et al.
Folia histochemica et cytobiologica, 43(1), 19-23 (2005-05-06)
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