E1257

Epidermaler Wachstumsfaktor aus Maussubmaxillardrüse

lyophilized powder, BioReagent, suitable for cell culture

• Synonym(e): EGF, Epidermis-Wachstumsfaktor
• CAS-Nummer: 62229-50-9
• EG-Nummer: 263-468-7
• MDL-Nummer: MFCD01634807
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse submaxillary glands
• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.02-2.0 ng/mL EC₅₀
• Verpackung: pkg of 0.1 mg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Farbe: white
• UniProt-Hinterlegungsnummer: P01132
• Lagertemp.: 2-8°C
• Angaben zum Gen: mouse ... Egf(13645)

Beschreibung

Allgemeine Beschreibung

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor. It is majorly expressed in kidney, parotid gland and salivary gland.

Anwendung

Epidermal Growth Factor from murine submaxillary gland has been used

• in studying its effects on the development of caustic esophageal stricture in rats
• for preparation of MCF-10A cell line
• for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)

Biochem./physiol. Wirkung

Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation. EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia. EGF plays a major role in the maintenance of hair and skin development.

Physikalische Form

Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.

Angaben zur Herstellung

Purified by gel filtration and ion-exchange chromatography.

Hinweis zur Analyse

The biological activity is measured in a proliferation assay using BALB/MK cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)