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Key Documents

D8515

Sigma-Aldrich

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA

lyophilized powder

Synonym(e):

DNA, Deoxyribonucleic acid, ds-DNA-Cellulose, DNA-Cellulose

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About This Item

MDL-Nummer:
MFCD00130925
UNSPSC-Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

Form

lyophilized powder

Kennzeichnungsgrad

3-8 mg double-stranded calf thymus DNA/g

Lagertemp.

−20°C

Allgemeine Beschreibung

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and the linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.

Anwendung

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.

Qualität

May contain a trace of Tris and EDTA.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Yi Zhou et al.
Journal of immunology (Baltimore, Md. : 1950), 198(7), 2568-2577 (2017-02-22)
CD74 mediates MHC class-II antigenic peptide loading and presentation and plays an important role in the pathogenesis of autoimmune diseases, including systemic lupus erythematosus. C57BL/6
Lutz Wobbe et al.
Nucleic acids research, 41(13), 6553-6567 (2013-05-08)
The molecular function of mTERFs (mitochondrial transcription termination factors) has so far only been described for metazoan members of the protein family and in animals they control mitochondrial replication, transcription and translation. Cells of photosynthetic eukaryotes harbour chloroplasts and mitochondria
S S Zhang et al.
The Journal of biological chemistry, 266(30), 20483-20490 (1991-10-25)
Two DNA helicases from calf thymus nuclei have been purified and characterized. The two proteins, designated as nuclear DNA helicase I and II, were copurified on Bio-Rex 70, DEAE-Sepharose, phosphocellulose and subsequently separated from each other on a heparin-Sepharose column.
A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
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