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Merck

D1033

Sigma-Aldrich

Monoclonal Anti-Desmin antibody produced in mouse

clone DE-U-10, ascites fluid

Synonym(e):

Anti-CDCD3, Anti-CSM1, Anti-CSM2, Anti-LGMD1D, Anti-LGMD1E, Anti-LGMD2R

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

DE-U-10, monoclonal

Mol-Gew.

antigen 50-55 kDa

Enthält

15 mM sodium azide

Speziesreaktivität

rabbit, chicken, hamster, feline, lizard, sheep, rat, human, bovine, viper, mouse, goat

Methode(n)

indirect immunofluorescence: 1:40 using formalin-fixed, paraffin-embedded sections of human tissue
microarray: suitable
western blot: 1:200 using chicken gizzard extract/whole extract of mouse myoblast C2C12 cells

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Des(13346)
rat ... Des(64362)

Allgemeine Beschreibung

Desmin is the protein subunit of muscle-type intermediate filaments and is found predominantly in skeletal, cardiac, and smooth muscle.
Monoclonal Anti-Desmin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The desmin protein contains a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. Desmin is is present in the periphery of Z-disk in smooth and striated muscles.

Spezifität

The antibody localizes desmin in cultured cells and tissue sections from several species and in tumors derived from muscle tissue (leiomyomas, rhabdomyosarcomas).

Immunogen

desmin from pig stomach

Anwendung

Monoclonal Anti-Desmin antibody produced in mouse has been used in:
  • immunohistochemical staining
  • immunoblot analyses
  • immunocytochemistry
  • immunocytochemical localization of intermediate filaments

Monoclonal Anti-Desmin antibody produced in mouse is suitable for indirect immunofluorescence at a working dilution of 1:20 using formalin-fixed, paraffin-embedded sections of human tissue, microarray and western blotting. It may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs. It specifically stains desmin when used in immunoblotting assays. It may be used for labelling desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas). It was used for immunohistochemical staining of cardiac cells in a study to understand cardiac conduction through engineered tissue. It was used as a primary antibody for the detection of expression of desmin in cultured cells to study the effect of mutation in desmin gene.

Biochem./physiol. Wirkung

Desmin provides mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Mutations in this gene damages the stability of intermediary filaments in cardiomyocytes.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Empfehlung

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

K Rosenvold et al.
Journal of animal science, 88(5), 1830-1841 (2010-02-02)
The current pork slaughter process is primarily optimized to reduce cooler shrink and the incidence of PSE pork. Elimination of the halothane gene and improved preslaughter handling have decreased the incidence of PSE pork and improved the water-holding capacity of
Andreas Brodehl et al.
Circulation. Cardiovascular genetics, 6(6), 615-623 (2013-11-10)
The intermediate filament protein desmin is encoded by the gene DES and contributes to the mechanical stabilization of the striated muscle sarcomere and cell contacts within the cardiac intercalated disk. DES mutations cause severe skeletal and cardiac muscle diseases with
Functional characterization of the novel DES mutation p. L136P associated with dilated cardiomyopathy reveals a dominant filament assembly defect
Brodehl A, et al.
Journal of Molecular and Cellular Cardiology, 91, 207-214 (2016)
Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration
Park S, et al.
International Journal of Molecular Medicine, 37(5), 1209-1220 (2016)
Cardiac conduction through engineered tissue
Choi YH, et al.
The American Journal of Pathology, 169(1), 72-85 (2006)

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